![]() ![]() Here we develop a mathematical model, termed the Common Base Method, for analysis of qPCR data based on threshold cycle values ( C q) and efficiencies of reactions ( E). Procedures for conducting qPCR have received significant attention however, more rigorous approaches to the statistical analysis of qPCR data are needed. QPCR has established itself as the technique of choice for the quantification of gene expression. ![]()
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